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High-throughput discovery of peptide activators of a bacterial sensor kinase

Brink KR, Mu AM, Hoang KV, Groszman K, Gunn JS, Tabor JJ
bioRxiv (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
170006pAM002Encodes PpmrD-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
170007pAM003Encodes PmgtC-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
170008pAM004Encodes PugtL-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
170009pAM005Encodes PvirK-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
170010pAM006Encodes PpagC-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
170011pAM007Encodes PslyB-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP
170012pKB222Encodes LacI-controlled expression of surface-displayed LL-37 and PvirK-mNeonGreen reporter
170013pKB223Encodes LacI-controlled expression of surface-displayed C18G and PvirK-mNeonGreen reporter
170014pKB224Encodes LacI-controlled expression of surface-displayed NC and PvirK-mNeonGreen reporter
170015pKB225Encodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporter
170016pKB226Encodes LacI-controlled expression of surface-displayed defensin HNP-1 and PvirK-mNeonGreen reporter
170017pKB230Encodes LacI-controlled expression of surface-displayed protegrin-1 and PvirK-mNeonGreen reporter
170018pKB231Encodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporter
170019pKB232Encodes LacI-controlled expression of surface-displayed murine CRAMP and PvirK-mNeonGreen reporter
170021pKB234Encodes LacI-controlled expression of surface-displayed hBD3 and PvirK-mNeonGreen reporter
170022pKB234.1Encodes LacI-controlled expression of surface-displayed hBD3 alpha-helix fragment and PvirK-mNeonGreen reporter
170023pKB234.2Encodes LacI-controlled expression of surface-displayed hBD3 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter
170024pKB235Encodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporter
170025pKB235.2Encodes LacI-controlled expression of surface-displayed CCL20 alpha-helix fragment and PvirK-mNeonGreen reporter
170026pKB235.4Encodes LacI-controlled expression of surface-displayed CCL20 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter
170027pKB236Encodes LacI-controlled expression of surface-displayed TCP and PvirK-mNeonGreen reporter
170028pKB236.1Encodes LacI-controlled expression of surface-displayed TCP fragment lacking C-terminal alpha-helix sequence and PvirK-mNeonGreen reporter
170029pKB236.2Encodes LacI-controlled expression of surface-displayed TCP C-terminal alpha-helix fragment and PvirK-mNeonGreen reporter
170030pKB237Encodes LacI-controlled expression of surface-displayed CCL19 and PvirK-mNeonGreen reporter
170031pKB237.1Encodes LacI-controlled expression of surface-displayed CCL19 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter
170032pKB237.2Encodes LacI-controlled expression of surface-displayed CCL19 alpha-helix fragment and PvirK-mNeonGreen reporter
170033pKB238Encodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporter
170034pKB239Encodes LacI-controlled expression of surface-displayed GNLY and PvirK-mNeonGreen reporter
170035pKB252Encodes LacI-controlled expression of surface-displayed K6L9 and PvirK-mNeonGreen reporter
170036pKB253Encodes LacI-controlled expression of surface-displayed K5L7 and PvirK-mNeonGreen reporter
170037pKB254Encodes LacI-controlled expression of surface-displayed H6L9 and PvirK-mNeonGreen reporter
170038pKB255Encodes LacI-controlled expression of surface-displayed melittin and PvirK-mNeonGreen reporter
170039pKB256Encodes LacI-controlled expression of surface-displayed Bac2a and PvirK-mNeonGreen reporter
170040pKB233Encodes PvirK-mNeonGreen reporter
179998pKB233.1Encodes Pmig-14-mNeonGreen reporter
179999pKB233.2Encodes PmgtA-mNeonGreen reporter
180000pKB233.3Encodes PpmrD-mNeonGreen reporter
180001pKB233.4Encodes PslyB-mNeonGreen reporter

Antibodies from Article