Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment.
Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, Bachovchin DA, Wu H
Immunity. 2021 Jul 13;54(7):1392-1404.e10. doi: 10.1016/j.immuni.2021.04.024. Epub 2021 May 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169948 | pcDNA3.1 LIC 6A CARD8-FLAG | Mammalian expression of CARD8-FLAG |
| 169949 | pcDNA3.1 LIC 6A CARD8-FLAG S297A | Mammalian expression of CARD8-FLAG that is autoprocessing-deficient (S297A) |
| 169950 | pcDNA3.1 LIC 6A CARD8-FLAG E274R | Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R) |
| 169951 | pcDNA3.1 LIC 6A CARD8-FLAG E274R + S297A | Mammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R) with an additional autoprocessing mutation (S297A) |
| 169952 | pcDNA3.1 LIC 6A CARD8-FLAG L368G | Mammalian expression of CARD8-FLAG with a site III mutation (L368G) |
| 169953 | pcDNA3.1 LIC 6A CARD8-FLAG F370G | Mammalian expression of CARD8-FLAG with a site III mutation (F370G) |
| 169954 | pcDNA3.1 LIC 6A CARD8-FLAG R394E | Mammalian expression of CARD8-FLAG with a site III mutation (R394E) |
| 169955 | pcDNA3.1 LIC 6A CARD8-FLAG R394E + S297A | Mammalian expression of CARD8-FLAG with a site III mutation (R394E) with an additional autoprocessing mutation (S297A) |
| 169956 | pcDNA3.1 LIC 6A CARD8-FLAG F405G | Mammalian expression of CARD8-FLAG with a site III mutation (F405G) |
| 169957 | pcDNA3.1 LIC 6A CARD8-FLAG F405G + S297A | Mammalian expression of CARD8-FLAG with a site III mutation (F405G) with an additional autoprocessing mutation (S297A) |
| 169958 | pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG | Mammalian expression of the CARD8 C-terminus fused to ubiquitin (co-translationally processed) |
| 169959 | pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG L368G | Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed) |
| 169960 | pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F370G | Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed) |
| 169961 | pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG R394E | Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed) |
| 169962 | pDONR221 CARD8 | pDONR221 for Gateway shuttling. Contains the CARD8 T60 isoform (no stop codon) |
| 169963 | pDONR221 CARD8 FIIND | pDONR221 for Gateway shuttling. Contains the FIIND (L162M-P434, no stop codon) |
| 169964 | pDONR221 CARD8 ZUC | pDONR221 for Gateway shuttling. Contains the CARD8 FIIND and CARD (L162M-L537, no stop codon) |
| 169965 | pDONR221 CARD8 Ub-S297 | pDONR221 for Gateway shuttling. Contains the CARD8 C-terminus fused to ubiquitin (co-translationally processed) |
| 169966 | pDONR221 CARD8 FIIND S297A | pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) |
| 169967 | pDONR221 CARD8 FIIND E274R | pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R) |
| 169968 | pDONR221 CARD8 FIIND E274R + S297A | pDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R) |
| 169969 | pDONR221 CARD8 FIIND L368G | pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (L368G) |
| 169970 | pDONR221 CARD8 FIIND L370G | pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F370G) |
| 169971 | pDONR221 CARD8 FIIND R394E | pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E) |
| 169972 | pDONR221 CARD8 FIIND R394E + S297A | pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E) |
| 169973 | pDONR221 CARD8 FIIND F405G | pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G) |
| 169974 | pDONR221 CARD8 FIIND F405G + S297A | pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G) |
| 169975 | pDONR221 CARD8 PAM | pDONR221 for Gateway shuttling. Contains CARD8 (no stop codon) with sgRNA binding site mutations |
| 169976 | pDONR221 CARD8 PAM S297A | pDONR221 for Gateway shuttling. Contains CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations |
| 169977 | pDONR221 CARD8 PAM E274R | pDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations |
| 169978 | pDONR221 CARD8 PAM L368G | pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (L368G) with sgRNA binding site mutations |
| 169979 | pDONR221 CARD8 PAM F370G | pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F370G) with sgRNA binding site mutations |
| 169980 | pDONR221 CARD8 PAM R394E | pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (R394E) with sgRNA binding site mutations |
| 169981 | pDONR221 CARD8 PAM F405G | pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F405G) with sgRNA binding site mutations |
| 169982 | pInducer20 CARD8-HA PAM | Tet/Dox-inducible mammalian expression of CARD8 with sgRNA binding site mutations |
| 169983 | pInducer20 CARD8-HA PAM S297A | Tet/Dox-inducible mammalian expression of CARD8 that is autoprocessing-deficient (S297A) with sgRNA binding site mutations |
| 169984 | pInducer20 CARD8-HA PAM E274R | Tet/Dox-inducible mammalian expression of CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutations |
| 169985 | pInducer20 CARD8-HA PAM L368G | Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (L368G) with sgRNA binding site mutations |
| 169986 | pInducer20 CARD8-HA PAM F370G | Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F370G) with sgRNA binding site mutations |
| 169987 | pInducer20 CARD8-HA PAM R394E | Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (R394E) with sgRNA binding site mutations |
| 169988 | pInducer20 CARD8-HA PAM F405G | Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F405G) with sgRNA binding site mutations |
| 169989 | pLEX305-N-dTAG-CARD8 | Mammalian expression of the dTAG-CARD8 fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1) |
| 169990 | pLEX305-N-dTAG-CARD8-ZUC | Mammalian expression of the dTAG-CARD8 ZUC (L162M-L537) fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1). |
| 169991 | pcDNA3.1 CARD8-TEV-GFP-FLAG | Mammalian purification construct for CARD8 |
| 169992 | pcDNA3.1 CARD8-TEV-GFP-FLAG S297A | Mammalian purification construct for CARD8 with an autoprocessing-deficient mutation |
| 170119 | pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG F405G | Mammalian expression of the CARD8 C-terminus with an interface III mutation fused to ubiquitin (co-translationally processed). |