Enhancing site-specific DNA integration by a Cas9 nuclease fused with a DNA donor-binding domain.
Ma S, Wang X, Hu Y, Lv J, Liu C, Liao K, Guo X, Wang D, Lin Y, Rong Z
Nucleic Acids Res. 2020 Oct 9;48(18):10590-10601. doi: 10.1093/nar/gkaa779.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169919 | pCAG-spCas9-SB | Expression of SpCas9-SB100X transposase fusion protein to enhace site-specific DNA integration. |
| 169920 | pCAG-spCas9-N57 | Expression of SpCas9-N57 fusion protein to enhace site-specific DNA integration. |
| 169921 | pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB | DNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins. |