MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo.
Zhang X, Lv S, Luo Z, Hu Y, Peng X, Lv J, Zhao S, Feng J, Huang G, Wan QL, Liu J, Huang H, Luan B, Wang D, Zhao X, Lin Y, Zhou Q, Zhang ZN, Rong Z
Nucleic Acids Res. 2021 Mar 22. pii: 6179929. doi: 10.1093/nar/gkab174.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169910 | CMV-VPR-S-L1-dCjCas9 MiniCAFE | Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression. |
| 169912 | pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D | Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9. |
| 169913 | pRGEN-CMV-VPR-L1-CjCas9 V-D | Expression of a VPR-dCjCas9 fusion protein to activate gene expression. |
| 169914 | pRGEN-CMV-VPR-WT CjCas9 V-WT | Expression of VPR-WT CjCas9 fusion protein to induce orthogonal genes knock out and activation. |
| 169915 | pU6-cj-E sgRNA | Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex. |
| 170115 | Pdpy-30-miniCAFE | Dpy-30 promoter-driven expression of a truncated VPR-dCjCas9 fusion protein (MiniCAFE) for activation of gene expression. |