A multiplex guide RNA expression system and its efficacy for plant genome engineering.
Oh Y, Lee B, Kim H, Kim SG
Plant Methods. 2020 Mar 12;16:37. doi: 10.1186/s13007-020-00580-x. eCollection 2020.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169765 | pECO200 | Express SpCas9 and Basta resistance gene |
| 169766 | pECO300 | Express SpCas9 and Kanamycin resistance gene |
| 169778 | pGRNA1 | Insert one gRNA |
| 169779 | pGRNA2 | Insert one gRNA |
| 169780 | pGRNA3 | Insert one gRNA |
| 169781 | pGRNA4 | Insert one gRNA |
| 169782 | pGRNA2e | Insert one gRNA |
| 169783 | pGRNA3e | Insert one gRNA |
| 169784 | pGRNA4e | Insert one gRNA |
| 169786 | pGRNA5e | Insert one gRNA |