phiC31 integrase for recombination-mediated single-copy insertion and genome manipulation in Caenorhabditis elegans.
Yang FJ, Chen CN, Chang T, Cheng TW, Chang NC, Kao CY, Lee CC, Huang YC, Hsu JC, Li J, Lu MJ, Chan SP, Wang J
Genetics. 2022 Feb 4;220(2):iyab206. doi: 10.1093/genetics/iyab206.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
169692 | pBRC_double_attB_GFP_donor | cargo vector for the phiC31 system with Psur5:GFP, cb_unc-119, and Gateway R1R2 site (for gene of interest insertion) |
169693 | pCG150_double_phiC31_attB | cargo vector for the phiC31 system with, unc-119 rescue fragment, no fluorescent marker. Gateway R3R4 sites (for gene of interest insertion) |