Saccharomyces cerevisiae Ecm2 Modulates the Catalytic Steps of pre-mRNA Splicing.
van der Feltz C, Nikolai B, Schneider C, Paulson JC, Fu X, Hoskins AA
RNA. 2021 Feb 5. pii: rna.077727.120. doi: 10.1261/rna.077727.120.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169922 | WT Ecm2 | Ecm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416 |
| 169923 | Ecm2 1-143 | WT Ecm2 mutated to introduce stop codon after AA 143 of Ecm2 |
| 169924 | Ecm2 1-197 | WT Ecm2 mutated to introduce stop codon after AA 197 of Ecm2 |
| 169925 | Ecm2 1-265 | WT Ecm2 mutated to introduce stop codon after AA 265 of Ecm2 |
| 169926 | Ecm2 1-325 | WT Ecm2 mutated to introduce stop codon after AA 325 of Ecm2 |
| 169927 | U6-U57A | WT U6 with a U57A mutation |
| 169928 | U6-U57C | WT U6 with a U57C mutation |