New tools for the visualization of glial fibrillary acidicprotein in living cells
Letra-Vilela R, Quiteres R, Murtinheira F, Crevenna A, Hensel Z, Herrera F
Experimental Results (2020), Volume 1, e4, 1–8
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169473 | human-GFAP-EGFP | We generated a human-GFAP-EGFP fusion construct by randomly inserting the coding region of EFGP into the coding region of hGFAP. |
| 169474 | mGFAP-Halo | the host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sites |
| 169475 | mGFAP R236H | Single p.Arg236His(R236H) mutation was individually inserted by site-directed mutagenesis into the wild-type mGFAP |
| 169476 | hGFAP R239C | Single p.Arg239Cys(R239C) mutations was individually inserted by site-directed mutagenesis into the wild-type hGFAP |