A synthetic circuit for buffering gene dosage variation between individual mammalian cells.

Yang J, Lee J, Land MA, Lai S, Igoshin OA, St-Pierre F
Nat Commun. 2021 Jul 5;12(1):4132. doi: 10.1038/s41467-021-23889-0. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
169367	Equalizer-H plasmid	Equalizer variant plasmid with high eGFP output levels but reduced gene dosage compensation capability.
169731	Equalizer-M plasmid	Equalizer variant plasmid with medium eGFP output levels and medium gene dosage compensation capability.
169732	Equalizer-L plasmid	Equalizer variant plasmid with low eGFP output levels but the best gene dosage compensation capability.
169733	Equalizer-H plasmid with onboard mCherry cassette	Equalizer-H plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
169734	Equalizer-M plasmid with onboard mCherry cassette	Equalizer-M plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
169735	Equalizer-L plasmid with onboard mCherry cassette	Equalizer-L plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
169737	Equalizer-L (mScarlet-I) plasmid with onboard mCitrine cassette	Equalizer-L plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.
169738	Equalizer-L episome	Equalizer-L on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
169739	CMV plasmid	Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels.
169740	CMV+ plasmid	Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.
169741	CMV+ plasmid with onboard mCherry cassette	CMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.
169742	CMV+ episome	CMV+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
169743	CMV+ (mScarlet-I) plasmid with onboard mCitrine cassette	CMV+ circuit plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.
169744	PGK plasmid	Unregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.
169745	PGK+ plasmid with onboard mCherry cassette	PGK+ plasmid that also encodes separate mCherry cassette. mCherry is used to monitor plasmid dosage.
169746	UBC plasmid	Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.
169747	Negative feedback (NF) plasmid	Standalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.
169748	Incoherent feedforward (IFF) plasmid	Standalone IFF circuit plasmid that has the same implementation of the IFF subcircuit in Equalizer-L.
170041	PGK+ episome	PGK+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.