Optimized CRISPR tools and site-directed transgenesis towards gene drive development in Culex quinquefasciatus mosquitoes.
Feng X, Lopez Del Amo V, Mameli E, Lee M, Bishop AL, Perrimon N, Gantz VM
Nat Commun. 2021 May 20;12(1):2960. doi: 10.1038/s41467-021-23239-0.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169238 | pVMG0146_Cq-U6-1_2xBbsI-gRNA | Expression of gRNA in Culex quinquefasciatus or other mosquitoes |
| 169323 | pVMG0149_Cq-U6-6_2xBbsI-gRNA | Expression of gRNA in Culex quinquefasciatus or other mosquitoes |
| 169339 | pVMG0217_Cq-U6-2b_2xBbsI-gRNA | Expression of gRNA in Culex quinquefasciatus or other mosquitoes |
| 169345 | pVMG0173_Cq-Actin5c-Cas9 | Expression of Cas9 in Culex quinquefasciatus or other mosquitoes |
| 169346 | pVMG0193_Cq-Rpl40-Cas9 | Expression of Cas9 in Culex quinquefasciatus or other mosquitoes |
| 169347 | pVMG0213_Cq-vasa-Cas9 | Expression of Cas9 in Culex quinquefasciatus or other mosquitoes |
| 169348 | pVMG0212_Cq-nanos-Cas9 | Expression of Cas9 in Culex quinquefasciatus or other mosquitoes |
| 169369 | pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop | Expression of gRNA in Culex quinquefasciatus or other mosquitoes |
| 169370 | pVMG0303_Cq-U6-6_2xBbsI_gRNA-Loop | Expression of gRNA in Culex quinquefasciatus or other mosquitoes |