Efficient and error-free fluorescent gene tagging in human organoids without double-strand DNA cleavage.

Bollen Y, Hageman JH, van Leenen P, Derks LLM, Ponsioen B, Buissant des Amorie JR, Verlaan-Klink I, van den Bos M, Terstappen LWMM, van Boxtel R, Snippert HJG
PLoS Biol. 2022 Jan 28;20(1):e3001527. doi: 10.1371/journal.pbio.3001527. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
169229	TVBB C-term-mNeongreen-Blast	Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Blast at the C-terminus of a target locus
169228	TVBB C-term-mNeongreen-Puro	Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Puro at the C-terminus of a target locus
169227	TVBB C-term-mNeongreen	Targeting vector backbone to support a knock-in of Linker-mNeongreen at the C-terminus of a target locus
169226	TVBB N-term-mNeongreen	Targeting vector backbone to support a knock-in of mNeongreen-Linker at the N-terminus of a target locus
169225	TVBB C-term-mTurquoise2-Hygro	Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Hygro at the C-terminus of a target locus
169224	TVBB C-term-mTurquoise2-Puro	Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locus
169223	TVBB C-term-mTurquoise2	Targeting vector backbone to support a knock-in of Linker-mTurquoise2 at the C-terminus of a target locus
169222	TVBB N-term-mTurquoise2	Targeting vector backbone to support a knock-in of mTurquoise2-Linker at the N-terminus of a target locus
169221	TVBB C-term-mScarlet-Blast	Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Blast at the C-terminus of a target locus
169220	TVBB C-term-mScarlet-Puro	Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Puro at the C-terminus of a target locus