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Force-Activated DNA Substrates for In Situ Generation of ssDNA and Designed ssDNA/dsDNA Structures in an Optical-Trapping Assay.

Taylor AMK, Okoniewski SR, Uyetake L, Perkins TT
Methods Mol Biol. 2022;2478:273-312. doi: 10.1007/978-1-0716-2229-2_10. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
169196hp-50%GC-20bpForce-activating hairpin for 3'-to-5' helicase
169197hp-50%GC-40bpForce-activating hairpin for 3'-to-5' helicase
169198hp-50%GC-40bp-RevbindsiteForce-activating hairpin for 5'-to-3' helicase
169199hp-66%GC-20bpForce-activating hairpin for 3'-to-5' helicase
169200hp-66%GC-40bpForce-activating hairpin for 3'-to-5' helicase
169201hp-75%GC-20bpForce-activating hairpin for 3'-to-5' helicase
169202hp-75%GC-40bpForce-activating hairpin for 3'-to-5' helicase
169203hp-100%GC-20bpForce-activating hairpin for 3'-to-5' helicase
169204hp-50%GC-Left-half-120bpForce-activating hairpin for 3'-to-5' helicase
169205hp-50%GC-Right-half-120bpForce-activating hairpin for 3'-to-5' helicase
169206hp-50-0-50%GC-Left-half-120bpForce-activating hairpin for 3'-to-5' helicase
169207hp-50-0-50%GC-Right-half-120bp50-0-50%GC-Right-half-120bp-hairpin

Antibodies from Article