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Lentiviral Capsid-Mediated Streptococcus pyogenes Cas9 Ribonucleoprotein Delivery for Efficient and Safe Multiplex Genome Editing.

Lu Z, Yao X, Lyu P, Yadav M, Yoo K, Atala A, Lu B
CRISPR J. 2021 Mar 16. doi: 10.1089/crispr.2020.0106. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
132553pspCas9-3'UTR-Tetra-com-vectorExpresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop
132555pspCas9-HF-tetra-com-vectorVector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.
136269pspCas9-3'UTR-ST2-com-vectorExpresses SpCas9 and sgRNA Scaffold

Antibodies from Article