Optimal Sacrificial Domains in Mechanical Polyproteins: S. epidermidis Adhesins Are Tuned for Work Dissipation.
Liu H, Liu Z, Yang B, Lopez Morales J, Nash MA
JACS Au. 2022 May 18;2(6):1417-1427. doi: 10.1021/jacsau.2c00121. eCollection 2022 Jun 27.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169133 | pET28a-SdrG-B1-ELP-HIS-ybbR | E. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements. |
| 169134 | pET28a-SdrG-B2-ELP-HIS-ybbR | E. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements. |
| 169135 | pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR | E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements. |
| 169136 | pET28a-Fgß-FIVAR-ELP-HIS-ybbR | E. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements. |
| 169137 | pET28a-VHH-ddFLN4-HIS-ybbR | E. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements. |
| 169138 | pET28a- mCherry-FIVAR-HIS-ybbR | E. coli expression of mCherry domain containing ybbR tag and FIVAR fingerprint domain. This construct was designed for AFM-SMFS measurements. |