A single-plasmid approach for genome editing coupled with long-term lineage analysis in chick embryos.
Gandhi S, Li Y, Tang W, Christensen JB, Urrutia HA, Vieceli FM, Piacentino ML, Bronner ME
Development. 2021 Mar 9. pii: dev.193565. doi: 10.1242/dev.193565.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169097 | pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI | Cas9 and Citrine expression in transfected cells |
| 169098 | HH-gRNA-HDV-Shuttle-Vector | Backbone plasmid to generate HH-gRNA-HDV segment |