Long-Range Optogenetic Control of Axon Guidance Overcomes Developmental Boundaries and Defects.
Harris JM, Wang AY, Boulanger-Weill J, Santoriello C, Foianini S, Lichtman JW, Zon LI, Arlotta P
Dev Cell. 2020 Jun 8;53(5):577-588.e7. doi: 10.1016/j.devcel.2020.05.009.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
169042 | pME-mCherry-PA-Rac1 | Middle entry vector for Gateway cloning that contains an mCherry fused with an optimized photoactivatable Rac1 gene insert |
169043 | p5E-3x125bp-mnx1 | 5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer. |
169044 | pDestTol2CG2-mnx1:mCherry-PA-Rac1 | Destination transgenesis vector for expressing mCherry fused with an optimized photoactivatable Rac1 protein in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer. |
169045 | pME-ReaChR-citrine | Middle entry vector for Gateway cloning that contains a ReaChR fused with citrine gene insert |
169046 | pDestTol2CG2-mnx1:ReaChR-citrine | Destination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer. |