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Long-Range Optogenetic Control of Axon Guidance Overcomes Developmental Boundaries and Defects.

Harris JM, Wang AY, Boulanger-Weill J, Santoriello C, Foianini S, Lichtman JW, Zon LI, Arlotta P
Dev Cell. 2020 Jun 8;53(5):577-588.e7. doi: 10.1016/j.devcel.2020.05.009. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
169042pME-mCherry-PA-Rac1Middle entry vector for Gateway cloning that contains an mCherry fused with an optimized photoactivatable Rac1 gene insert
169043p5E-3x125bp-mnx15' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.
169044pDestTol2CG2-mnx1:mCherry-PA-Rac1Destination transgenesis vector for expressing mCherry fused with an optimized photoactivatable Rac1 protein in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.
169045pME-ReaChR-citrineMiddle entry vector for Gateway cloning that contains a ReaChR fused with citrine gene insert
169046pDestTol2CG2-mnx1:ReaChR-citrineDestination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

Antibodies from Article