Heterologous biosynthesis of elsinochrome A sheds light on the formation of the photosensitive perylenequinone system.
Hu J, Sarrami F, Li H, Zhang G, Stubbs KA, Lacey E, Stewart SG, Karton A, Piggott AM, Chooi YH
Chem Sci. 2018 Nov 22;10(5):1457-1465. doi: 10.1039/c8sc02870b. eCollection 2019 Feb 7.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
168975 | pYFAC-riboB | Filamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; riboB |
168976 | pYFAC-pyroA | Filamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; pyroA |
168977 | pYFAC-CH1 | AMA1 based expression in filamentous fungi. pYFAC-pyrG carrying PalcA ; unique restriction site: PacI |
168978 | pYFAC-CH2 | AMA1 based expression in filamentous fungi pYFAC-pyrG carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA |
168979 | pYFAC-CH3 | AMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA |
168980 | pYFAC-CH4 | AMA1 based expression in filamentous fungi.pYFAC-pyroA carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA |