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Heterologous biosynthesis of elsinochrome A sheds light on the formation of the photosensitive perylenequinone system.

Hu J, Sarrami F, Li H, Zhang G, Stubbs KA, Lacey E, Stewart SG, Karton A, Piggott AM, Chooi YH
Chem Sci. 2018 Nov 22;10(5):1457-1465. doi: 10.1039/c8sc02870b. eCollection 2019 Feb 7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
168975pYFAC-riboBFilamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; riboB
168976pYFAC-pyroAFilamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; pyroA
168977pYFAC-CH1AMA1 based expression in filamentous fungi. pYFAC-pyrG carrying PalcA ; unique restriction site: PacI
168978pYFAC-CH2AMA1 based expression in filamentous fungi pYFAC-pyrG carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
168979pYFAC-CH3AMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
168980pYFAC-CH4AMA1 based expression in filamentous fungi.pYFAC-pyroA carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

Antibodies from Article