Determination of Rab5 activity in the cell by effector pull-down assay.
Qi Y, Liang Z, Wang Z, Lu G, Li G
Methods Mol Biol. 2015;1298:259-70. doi: 10.1007/978-1-4939-2569-8_22.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
168808 | pGEX-4T-2/Rabaptin-5:R5BD | Production of recombinant GST-R5BD (Rab5-binding domain) in bacteria for GST pulldown assays to identify binding partners for Rab5 subfamily members |