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Determination of Rab5 activity in the cell by effector pull-down assay.

Qi Y, Liang Z, Wang Z, Lu G, Li G
Methods Mol Biol. 2015;1298:259-70. doi: 10.1007/978-1-4939-2569-8_22. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
168808pGEX-4T-2/Rabaptin-5:R5BDProduction of recombinant GST-R5BD (Rab5-binding domain) in bacteria for GST pulldown assays to identify binding partners for Rab5 subfamily members

Antibodies from Article