Cre/lox-Mediated Chromosomal Integration of Biosynthetic Gene Clusters for Heterologous Expression in Aspergillus nidulans.
Roux I, Chooi YH
ACS Synth Biol. 2022 Feb 16. doi: 10.1021/acssynbio.1c00458.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 168784 | pGem-PgpdA-Cre-TtrpC | Helper vector for the transient expression of Cre recombinase in filamentous fungi. Not replicative in fungi. |
| 168785 | pGem-LP1-loxP-bar-lox2272-71 | Vector to create landing pad 1 (LP1) by homologous recombination in Aspergillus nidulans ΔSt site. The strain A. nidulans LP1 is used for recombinase mediated chromosomal integration. |
| 168786 | pGem-LP2-loxP-bar-lox2272-71 | Vector to create landing pad 2 (LP2) by homologous recombination in Aspergillus nidulans IS1 site. The strain A. nidulans LP2 is used for recombinase mediated chromosomal integration. |
| 168787 | pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66 | Donor vector for recombinase mediated integration in filamentous fungi. Is a shuttle vector for optional in vivo cloning in yeast, and contains four inducible promoters or sites for whole BGC cloning. |