Myristoylation alone is sufficient for PKA catalytic subunits to associate with the plasma membrane to regulate neuronal functions.

Xiong WH, Qin M, Zhong H
Proc Natl Acad Sci U S A. 2021 Apr 13;118(15). pii: 2021658118. doi: 10.1073/pnas.2021658118. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
168482	pCDNA3_PKA-CαN (G2A)-mVenus	Mammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.
168483	pCDNA3_PKA-CαN (K8,9A)-mVenus	Mammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.
168484	pCDNA3_PKA-CαN (K22,24A)-mVenus	Mammalian expression of the K22,24A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.
168485	pCDNA3_PKA-CαN (K29,30A)-mVenus	Mammalian expression of the K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.
168486	pCDNA3_PKA-CαN15-mVenus	Mammalian expression of the N-terminal 15 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.
168487	pCDNA3_PKA-CαN (K8,9E)-mVenus	Mammalian expression of the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.
168488	pCDNA3_PKA-CαN (E12,14K)-mVenus	Mammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.
168489	pCDNA3_PKA-RIIα-sREACh	Mammalian expression of mouse PKA regulatory subunit IIα C-terminally tagged by sREACh.
168490	pCDNA3_PKA-Cα(K8,9A)-mEGFP	Mammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.
168491	pCDNA3_shRNA resistant PKA-Cα(E12,14K)-mEGFP	Mammalian expression of the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
168492	pCDNA3_PKA-RIIα	Mammalian expression of mouse PKA regulatory subunit Iiα, untagged.
168493	pCDNA3_PKA-Cα(K8,9A)-mPAGFP	Mammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
168494	pCDNA3_PKA-CαN(K8,9A)-mPAGFP	Mammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.
168495	pCDNA3_PKA-CαN(E12,14K)-mPAGFP	Mammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.
168496	pCDNA3_PKA-CβN15-mVenus	Mammalian expression of the N-terminal 15 residues of mouse PKA catalytic subunit β C-terminally tagged by monomeric Venus.
168497	pCDNA3_dPKA-CAT1N17-mVenus	Mammalian expression of the N-terminal 17 residues of Drosophila PKA catalytic subunit 1 C-terminally tagged by monomeric Venus.
168498	pCDNA3_PKA-CαN (K8,9R)-mVenus	Mammalian expression of the K8,9R mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.
168499	pCDNA3_PKA-CαN (E12,14A)-mVenus	Mammalian expression of the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.