DiB-splits: nature-guided design of a novel fluorescent labeling split system.

Bozhanova NG, Gavrikov AS, Mishin AS, Meiler J
Sci Rep. 2020 Jul 6;10(1):11049. doi: 10.1038/s41598-020-67095-2. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
168472	pET11a-N-DiB2	N-fragment of the DiB2‐split protein. This plasmid needs to be co-transformed with Plasmid 168257: pMRBad-C-wtBlc to obtain DiB2‐split protein
168473	pET11a-Z-N-DiB2	N-fragment of the DiB2‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain DiB2‐split-Zip protein
168972	H2B-DiB2-splitN1–125	N-fragment of the DiB2‐split protein fused with histone H2B
168973	TagBFP-DiB2-splitN1–125	N-fragment of the DiB2‐split protein fused with blue fluorescent protein TagBFP
168974	TagBFP-DiB2-splitC110–177	C-fragment of the DiB2‐split protein fused with blue fluorescent protein TagBFP