Influence of the TorD signal peptide chaperone on Tat-dependent protein translocation.
Bageshwar UK, DattaGupta A, Musser SM
PLoS One. 2021 Sep 9;16(9):e0256715. doi: 10.1371/journal.pone.0256715. eCollection 2021.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 168516 | p-preSufI(IAC) | Modified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A. |
| 168517 | p-spTorA-GFP-H6C | GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension. |
| 169039 | pTorD-H6 | Escherichia coli TorD with C-terminal 6xHis in pET28a. |
| 169040 | pH6-spTorA-mCherry | mCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a. |
| 169041 | pH6-TEV-spTorA-GFP(C) | GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24. |