Endogenous promoter-driven sgRNA for monitoring the expression of low-abundance transcripts and lncRNAs.
Gao N, Hu J, He B, Ji Z, Hu X, Huang J, Wei Y, Peng J, Wei Y, Zhou Y, Shen X, Li H, Feng X, Xiao Q, Shi L, Sun Y, Zhou C, Zhou H, Yang H
Nat Cell Biol. 2021 Jan;23(1):99-108. doi: 10.1038/s41556-020-00610-9. Epub 2021 Jan 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 168290 | PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA | CRISPR-activator Suntag-P65-HSF1 (SPH) |
| 168291 | PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator | optimized miniCMV (OminiCMV) |
| 168305 | AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA | mediated knock-in of sgRNA precursor |