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Multiplexed CRISPR-Cas9 system in a single adeno-associated virus to simultaneously knock out redundant clock genes.

Kim B, Kim J, Chun M, Park I, Kwak D, Choi M, Kim K, Choe HK
Sci Rep. 2021 Jan 28;11(1):2575. doi: 10.1038/s41598-021-82287-0. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
164967CSAC-CrysExpresses sgRNA in mammalian cells
168281CSAC-PersExpresses sgRNA in mammalian cells
168296CSAC-Bmal1Expresses sgRNA in mammalian cells
171185sgLacZsExpresses sgRNA
176511sgCRY1Expresses sgRNA in mammalian cells
197457sgCRY2Expresses sgRNA(CRY2 gene) in mammalian cells

Antibodies from Article