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O-GlcNAc Engineering on a Target Protein in Cells with Nanobody-OGT and Nanobody-splitOGA.

Ramirez DH, Ge Y, Woo CM
Curr Protoc. 2021 May;1(5):e117. doi: 10.1002/cpz1.117. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
168188(365) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4)HA-tagged Ubc6E nanobody fused to a truncated OGT of 4 TPRs
168189(366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)HA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutation
168190(369) pcDNA3.1(+)-HA-nLaG6-(EAAAK)4-OGA(544-706)HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGA with residues 544-706. For use with myc-OGA(1-400) split-OGA system.
168191(424) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5, K852A)HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to an inactive truncated OGT of 4 TPRs with K852A mutation
168192(302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs

Antibodies from Article