O-GlcNAc Engineering on a Target Protein in Cells with Nanobody-OGT and Nanobody-splitOGA.
Ramirez DH, Ge Y, Woo CM
Curr Protoc. 2021 May;1(5):e117. doi: 10.1002/cpz1.117.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 168188 | (365) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4) | HA-tagged Ubc6E nanobody fused to a truncated OGT of 4 TPRs |
| 168189 | (366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A) | HA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutation |
| 168190 | (369) pcDNA3.1(+)-HA-nLaG6-(EAAAK)4-OGA(544-706) | HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGA with residues 544-706. For use with myc-OGA(1-400) split-OGA system. |
| 168191 | (424) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5, K852A) | HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to an inactive truncated OGT of 4 TPRs with K852A mutation |
| 168192 | (302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5) | HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs |