Dual modes of CRISPR-associated transposon homing.
Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, Macrae RK, Zhang F
Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 10.1016/j.cell.2021.03.006. Epub 2021 Mar 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 168133 | pMS1: pHelper(AvCAST)_AvPSP1 | Inducible expression of AvCAST proteins with crRNA targeting AvPSP1. |
| 168134 | pMS2: pHelper(AvCAST)_AvPSP1_ΔTniQ | Inducible expression of AvCAST proteins (except TniQ) with crRNA targeting AvPSP1. |
| 168135 | pMS3: pHelper(AvCAST)_AvPSP1_ΔTnsD | Inducible expression of AvCAST proteins (except TnsD) with crRNA targeting AvPSP1. |
| 168137 | pMS4: pHelper(AvCAST)_entry | Inducible expression of AvCAST proteins. Two BsaI sites for spacer cloning. |
| 168138 | pMS5: pHelper(AvCAST)_entry_ΔTniQ | Inducible expression of AvCAST proteins (except TniQ). Two BsaI sites for spacer cloning. |
| 168139 | pMS6: pHelper(AvCAST)_entry_ΔTnsD | Inducible expression of AvCAST proteins (except TnsD). Two BsaI sites for spacer cloning. |
| 168140 | pMS7: pCascade(AvCAST)_entry | Inducible expression of AvCAST Cascade proteins. Two BsaI sites for spacer cloning. |
| 168141 | pMS8: pTns(AvCAST) | Inducible expression of AvCAST TnsA, TnsB, TnsC, TniQ and TnsD proteins. |
| 168142 | pMS9: pTns(AvCAST)_ΔTniQ | Inducible expression of AvCAST TnsA, TnsB, TnsC and TnsD proteins. |
| 168143 | pMS10: pTns(AvCAST)_ΔTnsD | Inducible expression of AvCAST TnsA, TnsB, TnsC and TniQ proteins. |
| 168144 | pMS11: pTns(AvCAST)_ΔTniQ,ΔTnsD | Inducible expression of AvCAST TnsA, TnsB and TnsC proteins. |
| 168145 | pMS12: pDonor(AvCAST) | AvCAST donor harboring non-functional cargo. |
| 168146 | pMS13: pTarget(AvCAST) | Target plasmid containing AvPSP1 and attachement site for AvCAST. |
| 168147 | pMS14: pTwinStrep-SUMO-AvTnsA | AvTnsA expression construct for protein purification. |
| 168148 | pMS15: pTwinStrep-SUMO-AvTnsB | AvTnsB expression construct for protein purification. |
| 168149 | pMS16: pTwinStrep-SUMO-AvTnsC | AvTnsC expression construct for protein purification. |
| 168150 | pMS17: pTwinStrep-SUMO-AvTnsD | AvTnsD expression construct for protein purification. |
| 168151 | pMS18: pHelper(PmcCAST)_PmcPSP1 | Inducible expression of PmcCAST proteins with crRNA targeting PmcPSP1. |
| 168152 | pMS19: pHelper(PmcCAST)_PmcPSP1_ΔTniQ | Inducible expression of PmcCAST proteins (except TniQ) with crRNA targeting PmcPSP1. |
| 168153 | pMS20: pHelper(PmcCAST)_PmcPSP1_ΔTnsD | Inducible expression of PmcCAST proteins (except TnsD) with crRNA targeting PmcPSP1. |
| 168154 | pMS21: pHelper(PmcCAST)_entry | Inducible expression of PmcCAST proteins. Two BsaI sites for spacer cloning. |
| 168155 | pMS22: pHelper(PmcCAST)_entry_ΔTniQ | Inducible expression of PmcCAST proteins (except TniQ). Two BsaI sites for spacer cloning. |
| 168156 | pMS23: pHelper(PmcCAST)_entry_ΔTnsD | Inducible expression of PmcCAST proteins (except TnsD). Two BsaI sites for spacer cloning. |
| 168157 | pMS24: pCascade(PmcCAST)_entry | Inducible expression of PmcCAST Cascade proteins. Two BsaI sites for spacer cloning. |
| 168158 | pMS25: pTns(PmcCAST) | Inducible expression of PmcCAST TnsAB, TnsC, TniQ and TnsD proteins. |
| 168159 | pMS26: pTns(PmcCAST)_ΔTniQ | Inducible expression of PmcCAST TnsAB, TnsC and TnsD proteins. |
| 168160 | pMS27: pTns(PmcCAST)_ΔTnsD | Inducible expression of PmcCAST TnsAB, TnsC and TniQ proteins. |
| 168161 | pMS28: pTns(PmcCAST)_ΔTniQ,ΔTnsD | Inducible expression of PmcCAST TnsAB and TnsC proteins. |
| 168162 | pMS29: pDonor(PmcCAST) | PmcCAST donor harboring non-functional cargo. |
| 168163 | pMS30: pTarget(PmcCAST) | Target plasmid containing PmcPSP1 and attachment site for PmcCAST. |
| 168164 | pMS31: pTwinStrep-SUMO-PmcTnsAB | PmcTnsAB expression construct for protein purification. |
| 168165 | pMS32: pTwinStrep-SUMO-PmcTnsC | PmcTnsC expression construct for protein purification. |
| 168166 | pMS33: pTwinStrep-SUMO-PmcTnsD | PmcTnsD expression construct for protein purification. |