Induction of an immortalized songbird cell line allows for gene characterization and knockout by CRISPR-Cas9.
Biegler MT, Fedrigo O, Collier P, Mountcastle J, Haase B, Tilgner HU, Jarvis ED
Sci Rep. 2022 Mar 14;12(1):4369. doi: 10.1038/s41598-022-07434-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 168106 | pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE | PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning |
| 168107 | pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE | PiggyBac transposon vector constitutively driving PuroR-eGFP and conditionally expressing S.aureus Cas9 and mCherry |
| 168108 | pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE | Tol2 transposon vector of empty U6-S.aureus Cas9 gRNA cassette and CAG-driven Cre-NeoR |
| 168109 | pMB204: UbC-GFP-Tg_SAP30L-WPRE | Lentiviral expression of Zebra Finch SAP30L fused to the C-terminal end of eGFP |