Induction of an immortalized songbird cell line allows for gene characterization and knockout by CRISPR-Cas9.

Biegler MT, Fedrigo O, Collier P, Mountcastle J, Haase B, Tilgner HU, Jarvis ED
Sci Rep. 2022 Mar 14;12(1):4369. doi: 10.1038/s41598-022-07434-7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
168109	pMB204: UbC-GFP-Tg_SAP30L-WPRE	Lentiviral expression of Zebra Finch SAP30L fused to the C-terminal end of eGFP
168108	pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE	Tol2 transposon vector of empty U6-S.aureus Cas9 gRNA cassette and CAG-driven Cre-NeoR
168107	pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE	PiggyBac transposon vector constitutively driving PuroR-eGFP and conditionally expressing S.aureus Cas9 and mCherry
168106	pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE	PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning