Target protein deglycosylation in living cells by a nanobody-fused split O-GlcNAcase.
Ge Y, Ramirez DH, Yang B, D'Souza AK, Aonbangkhen C, Wong S, Woo CM
Nat Chem Biol. 2021 Mar 8. pii: 10.1038/s41589-021-00757-y. doi: 10.1038/s41589-021-00757-y.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 168094 | (81) pcDNA3.1-BC2-Nup62-Flag-EPEA | Nucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags |
| 168095 | (294) pcDNA3.1-myc-OGA(1-400) | Myc tagged truncated human OGA catalytic domain of residues 1-400 |
| 168096 | (318) pcDNA3.1-myc-OGA(1-400)D174N | Myc tagged inactive truncated human OGA catalytic domain of residues 1-400 with D174N mutation |
| 168097 | (344) pcDNA3.1-HA-nGFP-(EAAAK)4-OGA(544-706) | HA- tagged GFP nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA. |
| 168098 | (363) pcDNA3.1-Nup62-Ubc-Flag-EPEA | Nucleoporin Nup62 with C-terminal Ubc nanobody tag, Flag tag, and EPEA nanobody tag |
| 168099 | (367) pcDNA3.1-HA-nUbc-(EAAAK)4-OGA(544-706) | HA- tagged Ubc6E nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA |
| 168100 | (383) pcDNA3.1-HA-nBC2-OGA(544-706) | HA- tagged BC2 nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA |
| 168101 | (112) pcDNA3.1-GFP-Flag-Nup62-EPEA | GFP and Flag tagged Nup62 with EPEA nanobody tag |
| 168102 | (271) pcDNA3.1-HA-nEPEA-(EAAAK)3-OGA(544-706) | HA- tagged EPEA nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA |