Mapping Mechanostable Pulling Geometries of a Therapeutic Anticalin/CTLA-4 Protein Complex.

Liu Z, Moreira RA, Dujmovic A, Liu H, Yang B, Poma AB, Nash MA
Nano Lett. 2022 Jan 12;22(1):179-187. doi: 10.1021/acs.nanolett.1c03584. Epub 2021 Dec 17. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
168047	pET28a-SdrG-FLN-ELP-His-ybbr	Expresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus
168046	pET28a-anticalin (E143amb)-His	Expresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).
168045	pET28a-anticalin (N116amb)-His	Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).
168044	pET28a-anticalin (S87amb)-His	Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).
168043	pET28a-anticalin (E60amb)-His	Expresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).
168042	pET28a-anticalin (I55amb)-His	Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).
168041	pET28a-anticalin (N21amb)-His	Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).
168040	pET28a-Fgβ-anticalin-His-ybbr	Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus
168039	pET28a-anticalin-FLN-ELP-His-ybbr	Expresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus
168038	pET28a-CTLA4-FLN-ELP-His-ybbr	Expresses CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus