A functional genetic toolbox for human tissue-derived organoids.

Sun D, Evans L, Perrone F, Sokleva V, Lim K, Rezakhani S, Lutolf M, Zilbauer M, Rawlins EL
Elife. 2021 Oct 6;10. pii: 67886. doi: 10.7554/eLife.67886. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

Showing 1 to 10 of 10 entries

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Search table:

ID	Plasmid	Purpose
167973	AAVS1-EF1a-mTagRFP-T-CAAX	AAVS1 targeting repair template
167972	SOX9-T2A-H2B-EGFP repair template	SOX9 reporter repair template
167971	TP63-T2A-Rox-EF1a-Rox-Venus-NLS repair template	Tp63 reporter targeting repair template
167969	SOX2 KO (T2A-H2B-EGFP)	SOX2 CDS replacement with T2A-H2B-EGFP
167968	CMV-Dre-T2A-TagRFP	Transient Dre expression
167943	SFTPC-T2A-Rox-EF1a-Rox-Venus-NLS repair template	SFTPC reporter targeting repair template
167942	pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G	Inducible NKX2-1 overexpression
167937	pLenti-tetON-dxCas9(3.7)-VPR-EF1a-TagRFP-2A-tet3G	Inducible CRISPRa expression
167936	pLenti-U6-gRNA-EF1a-EGFP-CAAX	gRNA entry vector
167935	pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G	Inducible CRISPRi expression