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A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19.

Perveen S, Khalili Yazdi A, Devkota K, Li F, Ghiabi P, Hajian T, Loppnau P, Bolotokova A, Vedadi M
SLAS Discov. 2021 Jan 10:2472555220985040. doi: 10.1177/2472555220985040. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
167846p28BIOHTEV-LIC: nsp16_SARS-CoV-2Bacterial expression of the SARS-CoV-2 protein nsp16
167847p28BIOHTEV-LIC: nsp10_SARS-CoV-2Bacterial expression of the SARS-CoV-2 protein nsp10
167848p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA
167849pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA
167850pET28-MHL: nsp16_SARS-CoV-2Bacterial expression of the SARS-CoV-2 protein nsp16
167851pET28-MHL: nsp10_SARS-CoV-2Bacterial expression of the SARS-CoV-2 protein nsp10

Antibodies from Article