Quantifying single-cell ERK dynamics in colorectal cancer organoids reveals EGFR as an amplifier of oncogenic MAPK pathway signalling.
Ponsioen B, Post JB, Buissant des Amorie JR, Laskaris D, van Ineveld RL, Kersten S, Bertotti A, Sassi F, Sipieter F, Cappe B, Mertens S, Verlaan-Klink I, Boj SF, Vries RGJ, Rehmann H, Vandenabeele P, Riquet FB, Trusolino L, Bos JL, Snippert HJG
Nat Cell Biol. 2021 Apr;23(4):377-390. doi: 10.1038/s41556-021-00654-5. Epub 2021 Apr 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 167817 | miniTol2 x EF1a_EKAREN5 + PGK-puro | Optimized EKAREV FRET biosensor sensor for ERK |
| 167818 | miniTol2 x EF1a_EKAREN5 + PGK-blast | Optimized EKAREV FRET biosensor sensor for ERK |
| 167819 | miniTol2 x EF1a_EKAREN5(TA) + PGK-puro | control sensor for EKAREN5 |
| 167820 | miniTol2 x EF1a_EKAREN5(TA) + PGK-blast | control sensor for EKAREN5 |
| 167821 | pLV x EF1a_EKAREN5-ires-puro | Optimized EKAREV FRET biosensor sensor for ERK |
| 167822 | pLV x EF1a_EKAREN5-ires-blast | Optimized EKAREV FRET biosensor sensor for ERK |
| 167823 | pLV x EF1a_EKAREN5(TA)-ires-puro | control sensor for EKAREN5 |
| 167824 | miniTol2 x EF1a_EKAREN4 + PGK-puro | Optimized EKAREV FRET biosensor sensor for ERK |
| 167825 | miniTol2 x EF1a_EKAREN4 + PGK-blast | Optimized EKAREV FRET biosensor sensor for ERK |
| 167826 | miniTol2 x EF1a_EKAREN4(TA) + PGK-puro | Control sensor for EKAREN4 |
| 167827 | miniTol2 x EF1a_EKAREN4(TA) + PGK-blast | Control sensor for EKAREN4 |
| 167828 | pLV x EF1a_EKAREN4-ires-puro | Optimized EKAREV FRET biosensor sensor for ERK |
| 167829 | pLV x EF1a_EKAREN4-ires-blast | Optimized EKAREV FRET biosensor sensor for ERK |
| 167830 | pLV x EF1a_EKAREN4(TA)-ires-puro | Control sensor for EKAREN4 |
| 167831 | pLV x EF1a_EKAREN4(TA)-ires-blast | Control sensor for EKAREN4 |
| 167832 | pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro | Inducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLS |