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Adenine Base Editor Ribonucleoproteins Delivered by Lentivirus-Like Particles Show High On-Target Base Editing and Undetectable RNA Off-Target Activities.

Lyu P, Lu Z, Cho SI, Yadav M, Yoo KW, Atala A, Kim JS, Lu B
CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1089/crispr.2020.0095. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
132554pspCas9-ABE-3'UTR-sgRNA-2xMS2Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2
132560pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vectorVector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.
136270pspCas9-ABE-3'UTR-sgRNA-ST2-com vectorExpresses ABE and ST2-com modified sgRNA scaffold

Antibodies from Article