Cytoplasmic CPSF6 Regulates HIV-1 Capsid Trafficking and Infection in a Cyclophilin A-Dependent Manner.
Zhong Z, Ning J, Boggs EA, Jang S, Wallace C, Telmer C, Bruchez MP, Ahn J, Engelman AN, Zhang P, Watkins SC, Ambrose Z
mBio. 2021 Mar 23;12(2). pii: mBio.03142-20. doi: 10.1128/mBio.03142-20.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 153308 | pcDNA-IgKappa-myc-dL5- 2XG4S-GPI | Expresses GPI-anchored dL5** in mammalian cells. (FAP-GPI) |
| 167584 | pcDNA5-TO-Vpr-mRuby3-IN | For producing fluorescently labeled HIV-1; expresses HIV-1 integrase fused to Vpr for packaging into HIV-1 and the fluorophore mRuby3 |
| 167585 | pSICO-CPSF6-eGFP | Expresses human CPSF6 fused to eGFP |
| 167586 | pSICO-CPSF6-iRFP670 | Expresses human CPSF6 fused to iRFP |
| 167587 | pSICO-CPSF6-mNeonGreen | Expresses human CPSF6 fused to mNeonGreen |
| 167588 | pSICO-CPSF6-4Glu-iRFP670 | Expresses a mutant form of CPSF6 (CPSF6-4Glu described in Jang et al. Nucleic Acids Res 2019) fused to iRFP670 |
| 167590 | pSICO-eGFP-TNPO3 | Expresses human TNPO3 fused to eGFP |