RecT recombinase expression enables efficient gene editing in Enterococcus.

Chen V, Griffin ME, Maguin P, Varble A, Hang HC
Appl Environ Microbiol. 2021 Jul 7:AEM0084421. doi: 10.1128/AEM.00844-21. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
167546	pRecT_2	Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.
167547	pCas9	Used for CRISPR-Cas9 mediated recomineering in Enterococcus. Can clone desired gRNA using BsaI digestion.