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A dual conditional CRISPR-Cas9 system to activate gene editing and reduce off-target effects in human stem cells.

Park SB, Uchida T, Tilson S, Hu Z, Ma CD, Leek M, Eichner M, Hong SG, Liang TJ
Mol Ther Nucleic Acids. 2022 Apr 27;28:656-669. doi: 10.1016/j.omtn.2022.04.013. eCollection 2022 Jun 14. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
167500Lenti-Dual-iCas9-CMVExpression of dual inducible Cas9 with CMV promoter
167501Lenti-Dual-iCas9-EFSExpression of dual inducible Cas9 with EFS promoter
167502Lenti-Dual-iCas9-hALBExpression of dual inducible Cas9 with hALB promoter

Antibodies from Article