Records of RNA locations in living yeast revealed through covalent marks.
Medina-Munoz HC, Lapointe CP, Porter DF, Wickens M
Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23539-23547. doi: 10.1073/pnas.1921408117. Epub 2020 Sep 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 167452 | pHCMM1 | Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter |
| 167453 | pHCMM2 | Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter |
| 167454 | pHCMM3 | A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)] |
| 170359 | pHCMM4 | Expresses a Sec61p-mCherry ER-marker from the CYC1 promoter |