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Self-assembly of multi-component mitochondrial nucleoids via phase separation.

Feric M, Demarest TG, Tian J, Croteau DL, Bohr VA, Misteli T
EMBO J. 2021 Mar 15;40(6):e107165. doi: 10.15252/embj.2020107165. Epub 2021 Feb 23. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
167326Human_TFAM_HMGA_pET28Expresses TFAM HMGA mutant in bacterial cells
167327Human_TFAM_HMGA_linker_pET28Expresses TFAM HMGA+linker mutant in bacterial cells
167328Human_TFAM_dC_pET28Expresses TFAM dC-tail mutant in bacterial cells
167329Human_TFAM_dHMGA_pET28Expresses TFAM dHMGA mutant in bacterial cells
167330Human_TFAM_HMGB_ctail_pET28Expresses TFAM HMGB+C-tatil mutant in bacterial cells
167331pCDH_CMV_TFAM_mKate2Expresses mKate2 fused to full length TFAM in mammalian cells
167332pCDH_CMV_L6_mKate2Expresses mKate2 fused to TFAM L6 mutant in mammalian cells
167333pCDH_CMV_dimer_mKate2Expresses mKate2 fused to TFAM no-dimerization mutant in mammalian cells
167334pCDH_CMV_HMGA_mKate2Expresses mKate2 fused to TFAM HMGA mutant in mammalian cells
167335pCDH_CMV_HMGA_linker_mKate2Expresses mKate2 fused to TFAM HMGA+linker mutant in mammalian cells
167336pCDH_CMV_dC_mKate2Expresses mKatet2 fused to TFAM dC-tail mutant in mammalian cells
167337pCDH_CMV_dHMGA_mKate2_CLEAVAGEExpresses mKate2 fused to TFAM dHMGA mutant in mammalian cells
167338pCDH_CMV_HMGB_ctail_mKate2_CLEAVAGEExpresses mKate2 fused to TFAM HMGB+C-tail mutant in mammalian cells
167339pCDH_blast_MCS_Nard_GFP_D50Expresses GFP fused to progerin (d50) in mammalian cells
167340pCDH_blast_MCS_Nard_GFP_LAMINExpresses GFP fused to lamin A (LA) in mammalian cells

Antibodies from Article