Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag.
Wilhelm J, Kuhn S, Tarnawski M, Gotthard G, Tunnermann J, Tanzer T, Karpenko J, Mertes N, Xue L, Uhrig U, Reinstein J, Hiblot J, Johnsson K
Biochemistry. 2021 Aug 24;60(33):2560-2575. doi: 10.1021/acs.biochem.1c00258. Epub 2021 Aug 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 167266 | pET51b-His-TEV-HaloTag7 | Overexpression of HaloTag7 in E. coli |
| 167267 | pET51b-His-TEV-HaloTag7_dead_mt | Overexpression of the dead variant (D106A) of HaloTag7 in E. coli |
| 167268 | pET51b-His-TEV-HOB_(HaloTag7_oligo_binder) | Overexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coli |
| 167269 | pET51b-His-TEV-SNAP-tag | Overexpression of SNAP-tag in E. coli |
| 167270 | pET51b-His-TEV-SNAP-tag_dead_mt | Overexpression of the dead variant (C145A) of SNAP-tag in E. coli |
| 167271 | pET51b-His-TEV-SNAP-tag_fast | Overexpression of the fast variant (E30R) of SNAP-tag in E. coli |
| 167272 | pET51b-His-TEV-SNAP-tag_fast_dead_mt | Overexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coli |
| 167273 | pET51b-His-TEV-SNAP-tag_cx | Overexpression of crystallizing SNAP-tag variant (P179R) in E. coli |
| 167274 | pET51b-His-TEV-CLIP-tag | Overexpression of CLIP-tag in E. coli |
| 167275 | pET51b-His-TEV-CLIP-tag_fast | Overexpression of the fast variant (E30R) of CLIP-tag in E. coli |
| 167276 | pET51b-Strep-hAGT-His | Overexpression of the human o_-alkylguanine-dna alkyltransferase in E. coli |
| 167277 | pET51b-Strep-SsOGT-H5-His | Overexpression of a sulfolobal equivalent of SNAP-tag in E. coli |