Optimization of a T7-RNA polymerase system in Synechococcus sp. PCC 7002 mirrors the protein overproduction phenotype from E. coli BL21(DE3).
Jones CM, Korosh TC, Nielsen DR, Pfleger BF
Appl Microbiol Biotechnol. 2021 Feb;105(3):1147-1158. doi: 10.1007/s00253-020-11085-x. Epub 2021 Jan 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 215654 | pCMJ074 | pET28b derived T7 promoter with a 5’-UTR transcriptional fusion to clac143 |