A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing in Escherichia coli.

Li Q, Sun B, Chen J, Zhang Y, Jiang Y, Yang S
Acta Biochim Biophys Sin (Shanghai). 2021 Apr 15;53(5):620-627. doi: 10.1093/abbs/gmab036. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
73227	pEcCas	Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA
166581	pEcgRNA	CRISPR-Cas9-assisted genome editing in Escherichia coli