Efficient and iterative retron-mediated in vivo recombineering in Escherichia coli.
Ellington AJ, Reisch CR
Synth Biol (Oxf). 2022 May 3;7(1):ysac007. doi: 10.1093/synbio/ysac007. eCollection 2022.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166978 | pTJV1Sc-rpoB | Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance |
| 166979 | pTJV1ScGm-rpoB | Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aacC1 for gentamycin resistance |
| 166980 | pTJV1ScKm-rpoB | Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance |
| 166981 | pCas9CyMutL | Encodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repair |
| 166982 | pTJV1Sc2-tetA | Generates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance |
| 166983 | pTJV1Sc3-rpoB | Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance |