Harnessing the central dogma for stringent multi-level control of gene expression.
Greco FV, Pandi A, Erb TJ, Grierson CS, Gorochowski TE
Nat Commun. 2021 Mar 19;12(1):1738. doi: 10.1038/s41467-021-21995-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166922 | pMLC-BB1 | Backbone for assembly of multi-level gene expression controllers |
| 166923 | pA-01 | LacI expression cassette (A) |
| 166924 | pB-01 | Ptac promoter part (B) |
| 166925 | pC-01 | sRNA part (C) |
| 166926 | pC-02 | STAR-AD1.A5 part (C) |
| 166927 | pC-03 | STAR-AD1.A6 part (C) |
| 166928 | pC-04 | STAR-DC part (C) |
| 166929 | pD-01 | TrrnB and L3S2P21 terminator part (D) |
| 166930 | pE-01 | Stop codon part (E) |
| 166931 | pF-01 | GFP coding region part (F) |
| 166932 | pG-01 | Toehold switch part (G) |
| 166933 | pG-02 | Target AD1.A5 part (G) |
| 166934 | pG-03 | Target AD1.A6 part (G) |
| 166935 | pG-04 | Target DC (G) |
| 166936 | pH-01 | Reverse Ptac promoter part (H) |
| 166937 | pPtac | Ptac single-level gene expression controller (SLC) |
| 166938 | pTHS | Toehold switch multi-level gene expression controller (MLC) |
| 166939 | pSTAR | STAR-based multi-level gene expression controller (MLC) |
| 166940 | pSTAR2 | STAR-based multi-level gene expression controller (MLC) |
| 166941 | pDC | Dual control based multi-level gene expression controller (MLC) |