Engineering a conserved RNA regulatory protein repurposes its biological function in vivo.
Bhat VD, McCann KL, Wang Y, Fonseca DR, Shukla T, Alexander JC, Qiu C, Wickens M, Lo TW, Tanaka Hall TM, Campbell ZT
Elife. 2019 Jan 17;8. pii: 43788. doi: 10.7554/eLife.43788.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166972 | pSMT3-FBF2 | Expresses FBF-2 in E. coli for purification and crystallization studies |
| 166973 | pSMT3-FBF2 SS/Y | Expresses FBF-2 with SS/Y mutations in repeat 5 in E. coli for purification and crystallization studies |
| 166974 | pSMT3-FBF2 AS/Y | Expresses FBF-2 with AS/Y mutations in repeat 5 in E. coli for purification and crystallization studies |
| 166975 | pSMT3-FBF2 AQ/Y | Expresses FBF-2 with AQ/Y mutations in repeat 5 in E. coli for purification and crystallization studies |
| 166976 | pSMT3-FBF2 SS/R | Expresses FBF-2 with SS/R mutations in repeat 5 in E. coli for purification and crystallization studies |