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Stable integration of an optimized inducible promoter system enables spatiotemporal control of gene expression throughout avian development.

Chu D, Nguyen A, Smith SS, Vavrusova Z, Schneider RA
Biol Open. 2020 Oct 6;9(10). pii: bio.055343. doi: 10.1242/bio.055343. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
169895pNanoMinimal cloning vector
169897pEPIC1.1Empty constitutive expression vector with piggyBac, IRES, and mClover3
169898pPID2Minimal empty vector with piggyBac, insulators, and a DTS
169899pPIDCBEmpty dox inducible vector with piggyBac, insulators, and a DTS
169900pPIDCB2Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mClover3 is fused to H2B to allow for visualization of the nucleus.
169901pPIDNBEmpty dox inducible vector with piggyBac, insulators, and a DTS
169902pPIDNB2Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mNeonGreen is fused to H2B to allow for visualization of the nucleus.

Antibodies from Article