Methods for in vitro CRISPR/CasRx-Mediated RNA Editing.
Chuang YF, Wang PY, Kumar S, Lama S, Lin FL, Liu GS
Front Cell Dev Biol. 2021 Jun 11;9:667879. doi: 10.3389/fcell.2021.667879. eCollection 2021.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166867 | pM115: CasRx control presgRNA | U6-driven expression of control (non-targeting) presgRNA compatible with CasRx. |
| 166868 | pM116: CasRx VEGFA presgRNA | U6-driven expression of human VEGFA targeting presgRNA compatible with CasRx. |
| 166869 | pM117: VEGFA array presgRNA | U6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx. |
| 166870 | pM122: pAAV-EFS-CasRx | AAV vector for expressing CasRx |
| 166871 | pM123: pAAV-EFS-CasRx-control presgRNA | AAV vector for expressing CasRx and control presgRNA for RNA-editing |
| 166872 | pM124: pAAV-EFS-CasRx-VEGFA presgRNA | AAV vector for expressing CasRx and human VEGFA targeting presgRNA for RNA-editing |
| 166873 | pM125: pAAV-EFS-CasRx-VEGFA array presgRNA | AAV vector for expressing CasRx and human VEGFA targeting presgRNA array for RNA-editing |