Allosteric Activation of SARS-CoV-2 RNA-Dependent RNA Polymerase by Remdesivir Triphosphate and Other Phosphorylated Nucleotides.

Wang B, Svetlov V, Wolf YI, Koonin EV, Nudler E, Artsimovitch I
mBio. 2021 Jun 29;12(3):e0142321. doi: 10.1128/mBio.01423-21. Epub 2021 Jun 22. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
166860	pIA1362	Expression of codon optimized SARS-COV-2 Nsp7 in E. coli
166861	pIA1363	Expression of codon optimized SARS-COV-2 Nsp8 in E. coli
166862	pIA1385	Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.