The carboxy terminus of yeast Atg13 binds phospholipid membrane via motifs that overlap with the Vac8-interacting domain.
Gatica D, Damasio A, Pascual C, Klionsky DJ, Ragusa MJ, Popelka H
Autophagy. 2020 Jun;16(6):1007-1020. doi: 10.1080/15548627.2019.1648117. Epub 2019 Aug 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166852 | pCu-His6-Atg13[571–700]-Ss(424) | Expresses 6xHis tagged ATG13 [571–700] fragment with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast. |
| 166853 | pCu-His6-Atg13[571–700] F641G, I645G-Ss(424) | Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641G, I645G with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast |
| 166854 | pCu-His6-Atg13[571–700] F641E, I645E-Ss(424) | Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641E, I645E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast |
| 166855 | pCu-His6-Atg13[571–700] K683E, H685E, K686E-Ss(424) | Expresses 6xHis tagged ATG13 [571–700] fragment mutated in K683E, H685E, K686E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast |