Vac8 determines phagophore assembly site vacuolar localization during nitrogen starvation-induced autophagy.
Gatica D, Wen X, Cheong H, Klionsky DJ
Autophagy. 2020 Jun 8. doi: 10.1080/15548627.2020.1776474.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166836 | pRS405 VAC8-GFP | Expresses Vac8-GFP in yeasts. |
| 166837 | pRS405 VAC8-G2A-GFP | Expresses a myristoylation mutant Vac8-GFP in yeasts, mutation is Gly2Ala |
| 166838 | pRS405 VAC8-C4A,C5A,C7A-GFP | Expresses a palmitoylation mutant Vac8-GFP in yeasts, mutations are Cys4Ala, Cys5Ala, Cys7Ala |
| 166839 | pRS405 VAC8-G2A,C4A,C5A,C7A-GFP | Expresses a myristoylation and palmitoylation mutant Vac8-GFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7Ala |
| 166840 | pRS405 SEC66-VAC8-GFP | Expresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain. |
| 166841 | pRS405 VAC8-RFP | Expresses Vac8-RFP in yeasts |
| 166842 | pRS405 VAC8-G2A,C4A,C5A,C7A-RFP | Expresses a myristoylation and palmitoylation mutant Vac8-RFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7Ala |
| 166843 | pRS405 SEC66-VAC8-RFP | Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain. |
| 166844 | pRS405 PEX14-VAC8-RFP | Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF |