Efficient multiplexed gene regulation in Saccharomyces cerevisiae using dCas12a.
Ciurkot K, Gorochowski TE, Roubos JA, Verwaal R
Nucleic Acids Res. 2021 Jul 1. pii: 6312738. doi: 10.1093/nar/gkab529.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166728 | pCSN069 | Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiae |
| 166729 | pCSN071 | Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae |
| 166730 | pCSN072 | Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae |
| 166731 | pCSN074 | Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae |
| 166732 | pCSN075 | Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae |